DOT Observed Drug Testing Services
At On-Time Screening, we specialize in providing DOT Observed Drug Testing services to ensure compliance with the Department of Transportation (DOT) regulations for safety-sensitive positions. Whether you’re conducting pre-employment, random, post-accident, or return-to-duty testing, our professional observed drug testing services help maintain a safe, compliant, and drug-free workforce.
Why Choose On-Time Screening for DOT Observed Drug Testing?
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DOT-Compliant Testing: Our observed drug testing services fully comply with DOT regulations, including 49 CFR Part 40, ensuring you meet federal requirements for safety-sensitive employees in industries such as transportation, aviation, and public transit.
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Certified & Professional Technicians: Our trained and certified professionals perform the observed drug testing process in a respectful, secure, and confidential manner, following all DOT guidelines.
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Quick & Accurate Results: We offer fast turnaround times for test results, ensuring compliance deadlines are met and helping you make timely decisions regarding employee fitness for duty.
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Nationwide Coverage: With locations across the country and mobile testing options, we can provide convenient DOT observed testing wherever your workforce is located.

What is DOT Observed Drug Testing?
DOT observed drug testing involves a trained professional directly observing the employee during the urine specimen collection process. This is typically required under specific circumstances, including:
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Pre-Employment: When hiring individuals for safety-sensitive roles in DOT-regulated industries.
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Post-Accident: After an employee has been involved in an accident while performing safety-sensitive duties.
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Random Testing: Periodic, unannounced drug tests to ensure ongoing compliance with DOT regulations.
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Return-to-Duty & Follow-Up Testing: Required for employees returning to safety-sensitive duties after violating DOT drug and alcohol regulations.
How It Works
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Schedule the Test: Schedule your DOT-observed drug test online or by contacting our team. We offer flexibility with testing times and locations, including mobile testing services at your workplace.
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Observation Process: A trained technician will observe the employee during the urine specimen collection. The employee is asked to provide a urine sample in a private setting while the technician ensures the process follows DOT guidelines.
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Lab Analysis: Once the urine sample is collected, it is sent to a certified laboratory for analysis to test for a wide range of substances, including marijuana, cocaine, opiates, amphetamines, and more.
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Receive Results: Test results are securely delivered, with a fast turnaround time to ensure compliance with DOT deadlines and quick decision-making.
Benefits of DOT Observed Drug Testing
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Compliance with DOT Regulations: Our services ensure that your company stays fully compliant with the federal DOT regulations for drug testing, helping avoid penalties and legal issues.
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Enhanced Workplace Safety: By conducting DOT-observed drug tests, you ensure that safety-sensitive employees are free from impairment, reducing the risk of accidents and injuries on the job.
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Trustworthy & Professional Service: Our certified professionals provide a respectful and confidential testing experience, maintaining the integrity of the testing process.
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Convenient Nationwide Testing: With locations across the country and mobile testing options, we make it easy to implement DOT-observed drug testing wherever your workforce operates.